Composite

Part:BBa_K5062007:Design

Designed by: Christian Nelson Tejedor Vicera   Group: iGEM24_HKU-HongKong   (2024-09-27)


5th Generation Nanobody Chimeric Antigen Receptor (CAR) targeting GPC-3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2947
    Illegal SpeI site found at 1264
    Illegal PstI site found at 423
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2947
    Illegal SpeI site found at 1264
    Illegal PstI site found at 423
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2947
    Illegal BamHI site found at 54
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2947
    Illegal SpeI site found at 1264
    Illegal PstI site found at 423
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2947
    Illegal SpeI site found at 1264
    Illegal PstI site found at 423
    Illegal AgeI site found at 60
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1019
    Illegal BsaI.rc site found at 524
    Illegal BsaI.rc site found at 2231
    Illegal SapI.rc site found at 2436


Design Notes

The entire sequence is a large open reading frame that splits into several proteins upon translation. It is specifically designed for use in human cells


Source

Some are from genomic sequences, some are found from papers.

References